KMID : 0861020170320040039
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Korea Journal of Herbology 2017 Volume.32 No. 4 p.39 ~ p.46
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Antioxidant Activities of Hot Water and Ethanol Extracts from Portulacae Herba
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Park So-Ra
Han Ji-Woo Kang Ji-Young Kill Ki-Jung Yoo Ji-Hyun
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Abstract
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Objectives : This study aims to provide basic data about Portulacae Herba (PH) extracts as natural antioxidants by considering diverse antioxidant activities of PH depending on solvents.
Methods : The samples of PH were pulverized, and A hot water and a 70% EtOH were stir-extracted for two hours three times repeatedly in a water bath with a temperature of 95 degrees and at room temperature respectively to measure 7 kinds of antioxidant activities.
Results : There were significant differences in total phenol content, because the total phenol content of the 70% EtOH extract was higher than the hot water extract`s, and the total flavonoid content of the 70% EtOH extract(4.40 §¶/§·) was nearly 3.8 times higher than the hot water extract`s(1.16 §¶/§·). DPPH and ABTS radical scavenging activities were 70% EtOH extract showed a little higher activity than the hot water extract, and at a concentration of 500 §¶/§·, the highest scavenging activity was found in the 70% EtOH extract, not in the control group. Hydroxyl radical and Fe2+chelatingactivities were slightly higher in hot water extract than in 70% EtOH extract, and increased in a dose-dependent manner. Nitrite scavenging activities increased dose-dependently in the hot water and the 70% EtOH extract, regardless of the pH level, and scavenging activity of the 70% EtOH extract was higher at pH 1.2 than at pH 3.0.
Conclusions : In conclusion, it is thought that PH hot water and 70% EtOH extract have antioxidant activities, and can be used as natural antioxidants in future.
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KEYWORD
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Portulacae Herba, Antioxidant, Hot-Water Extract, Ethanol Extracts
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